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dc.contributor.authorCirak, C.
dc.contributor.authorRadusiene, J.
dc.contributor.authorKurtarc, E. S.
dc.contributor.authorMarksa, M.
dc.contributor.authorIvanauskas, L.
dc.date.accessioned2020-06-21T12:19:08Z
dc.date.available2020-06-21T12:19:08Z
dc.date.issued2020
dc.identifier.issn0254-6299
dc.identifier.issn1727-9321
dc.identifier.urihttps://doi.org/10.1016/j.sajb.2019.11.028
dc.identifier.urihttps://hdl.handle.net/20.500.12712/10353
dc.descriptionWOS: 000506413200037en_US
dc.description.abstractThe aim of the study was to introduce two endangered species from Turkish flora, Hypericum aviculariifolium and H. pruinatum, into in vitro culture and to determine the elicitation effect of jasmonic acid (JA) on the accumulation of secondary metabolites in vitro regenerated flowering plants. A Murashige and Skoog (MS) medium supplemented with plant growth regulators were used for seed germination and seedling culture. Seedling shoot explants in MS medium supplemented with combination of 1 mg/l BA and IAA resulted in maximum and simultaneous induction of roots and shoots. The regenerants of both species survived (98%) in greenhouse cultivation and were assayed for bioactive compounds. The content of secondary metabolites of flowering plants, originating from in vitro culture and treated with different doses of JA was higher than that of intact, i.e. maternal plants providing seeds and control plants. However, the intact and control plants did not differ significantly in the amounts of the bioactive compounds tested. The chemical profile of both species was similar with quantitative differences of individual compounds. Successful establishment of a highly efficient in vitro multiplication protocol in the present study offers a crucial in vitro strategy for the further conservation of endemic and rare species and enhanced production of valuable secondary metabolites. (C) 2019 SAAB. Published by Elsevier B.V. All rights reserved.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.sajb.2019.11.028en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGrowth regulatorsen_US
dc.subjectElicitationen_US
dc.subjectHyperforinen_US
dc.subjectHypericinen_US
dc.subjectHypericum spp.en_US
dc.subjectIn vitro cultureen_US
dc.subjectJasmonic aciden_US
dc.subjectPhenolic compoundsen_US
dc.titleIn vitro plant regeneration and jasmonic acid induced bioactive chemical accumulations in two Hypericum species from Turkeyen_US
dc.typearticleen_US
dc.contributor.departmentOMÜen_US
dc.identifier.volume128en_US
dc.identifier.startpage312en_US
dc.identifier.endpage318en_US
dc.relation.journalSouth African Journal of Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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