Yazar "Yurdusev, Nevzat" için listeleme
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Bi-antigenic immunoassay models based on the recombinant PvpA proteins for Mycoplasma gallisepticum diagnosis in chickens
Buyuktanir, Ozlem; Genc, Oktay; Yurdusev, Nevzat (Amer Assoc Veterinary Laboratory Diagnosticians Inc, 2010)The present study aimed to produce the relatively conserved central fragment of the Mycoplasma gallisepticum PvpA cytadhesin as recombinant antigen and to determine its species-specific diagnostic potential in comparison ... -
Detection of Brucella Antibody and DNA in Cow Milk by ELISA and PCR Methods
Terzi, Goknur; Buyuktanir, Ozlem; Genc, Oktay; Gucukoglu, Ali; Yurdusev, Nevzat (Kafkas Univ, Veteriner Fakultesi Dergisi, 2010)The aim of the present study was to determine comparatively the presence of anti-Brucella antibody and Brucella DNA in cow milk. Anti-Brucella antibody was detected by ELISA based on the lipopolysaccharide (LPS) as diagnostic ... -
Development of an individual rapid test based on enzymatic immunofiltration assay for detection of anti-Brucella abortus antibody in bovine sera
Genc, Oktay; Buyuktanir, Ozlem; Yurdusev, Nevzat (Sage Publications Inc, 2011)To detect bovine antibody directed to smooth lipopolysaccharide (LPS), cell lysate (LYS), O-polysaccharide (OPS), and LPS-deprived chromatographic fractions (ChF) of Brucella abortus, 2 bi-antigenic diagnostic models based ... -
Development of Qualitative and Quantitative ELISA Models for Bovine Brucellosis Diagnosis
Genc, Oktay; Buyuktanir, Ozlem; Yurdusev, Nevzat (Kafkas Univ, Veteriner Fakultesi Dergisi, 2010)In this study, a qualitative and a quantitative ELISA models (iELISA and qELISA, respectively) have been developed for screening and quantifying IgG isotype anti-Brucella abortus LPS antibody. For developing high sensitive ... -
Generation of Mouse Hybridomas Secreting Anti-Salmonella Enteritidis Antibodies and Their Preliminary Characterization
Buyuktanir, Ozlem; Yagci, Tamer; Findik, Arzu; Yildirim, Tuba; Yurdusev, Nevzat (Kafkas Univ, Veteriner Fakultesi Dergisi, 2011)BALB/c mice were intraperitoneally immunized with inactivated bacteria for generation of monoclonal anti-S. Enteritidis antibody. The spleen cells of the highest responder animal at fifth immunization were used as the ... -
LPS and Flagellin-Based Models for Serological Screening and Confirmation of Salmonella Infections in Chickens
Findik, Arzu; Buyuktanir, Ozlem; Yurdusev, Nevzat (Kafkas Univ, Veteriner Fakultesi Dergisi, 2010)Avian salmonellosis due to Salmonella B and D serogroups has a great importance because of the public health and the economical losses in poultry industry. For accurate diagnosis of avian salmonellosis, a total of 126 ... -
Production, Purification and Characterization of the Recombinant Brucella abortus rP17 Protein
Buyuktanir, Ozlem; Genc, Oktay; Yurdusev, Nevzat (Kafkas Univ, Veteriner Fakultesi Dergisi, 2011)Immunoreactive cytosolic P17 protein of Brucella abortus was produced in Escherichia coli as 6xHistidine tagged recombinant protein (rP17) by cloning the p17 gene into pColdI cold-shock expression vector. DNA sequence ... -
Rapid Immunofiltration Assay As A Field Diagnostic Tool For Ovine Brucellosis
Buyuktanir, Ozlem; Genc, Oktay; Celebi, Ozgur; Yurdusev, Nevzat (Taylor & Francis Inc, 2012)This work describes the development of two rapid immunofiltration assays, enzymatic (ERIFA) and non-enzymatic (NERIFA), for the rapid detection of ovine anti-Brucella antibodies. Brucella abortus lipopolysaccharide and ... -
Rapid immunofiltration assay based on colloidal gold-protein G conjugate as an alternative screening test for bovine and ovine brucellosis
Genc, Oktay; Buyuktanir, Ozlem; Yurdusev, Nevzat (Springer, 2012)A non-enzymatic rapid immunofiltration assay (NERIFA) was developed as an alternative field test for rapid detection of anti-Brucella antibody in bovine and ovine sera. The assay was based on Brucella abortus lipopolysaccharide ... -
A recombinant PvpA protein-based diagnostic prototype for rapid screening of chicken Mycoplasma gallisepticum infections
Bueyuektanir, Ozlem; Yildirim, Tuba; Yakicier, Cengiz; Genc, Oktay; Yurdusev, Nevzat (Elsevier Science Bv, 2008)Mycoplasma gallisepticum is the primary agent of chronic respiratory disease causing important economic losses in the poultry industry. Serological monitoring is essential to maintain mycoplasma-free breeder flocks and ... -
Stable transmission and transcription of Newfoundland ocean pout type III fish antifreeze protein (AFP) gene in transgenic mice and hypothermic storage of transgenic ovary and testis
Bagis, Haydar; Aktoprakligil, Digdem; Mercan, Hande Odaman; Yurdusev, Nevzat; Turgut, Gazi; Sekmen, Sakir; Cetin, Seyfettin (Wiley-Liss, 2006)Here we describe the generation of transgenic mice carrying type III fish antifreeze protein (AFP) gene and evaluate whether AFP type III protects transgenic mouse ovaries and testes from hypothermic storage. AFPs exist ...
