Yazar "Wallace, R. John" için listeleme
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Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant
McKain, Nest; Genc, Bugra; Snelling, Timothy J.; Wallace, R. John (Elsevier, 2013)Bacteria and archaea in frozen (-20 degrees C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes ... -
Diversity and Community Composition of Methanogenic Archaea in the Rumen of Scottish Upland Sheep Assessed by Different Methods
Snelling, Timothy J.; Genc, Bugra; McKain, Nest; Watson, Mick; Waters, Sinead M.; Creevey, Christopher J.; Wallace, R. John (Public Library Science, 2014)Ruminal archaeomes of two mature sheep grazing in the Scottish uplands were analysed by different sequencing and analysis methods in order to compare the apparent archaeal communities. All methods revealed that the majority ...