Yazar "Buyuktanir, Ozlem" için listeleme
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Bi-antigenic immunoassay models based on the recombinant PvpA proteins for Mycoplasma gallisepticum diagnosis in chickens
Buyuktanir, Ozlem; Genc, Oktay; Yurdusev, Nevzat (Amer Assoc Veterinary Laboratory Diagnosticians Inc, 2010)The present study aimed to produce the relatively conserved central fragment of the Mycoplasma gallisepticum PvpA cytadhesin as recombinant antigen and to determine its species-specific diagnostic potential in comparison ... -
Borrelia burgdorferi Methods and Protocols Preface
Pal, Utpal; Buyuktanir, Ozlem (Humana Press Inc, 2018)… -
Cross-Species Interferon Signaling Boosts Microbicidal Activity within the Tick Vector
Smith, Alexis A.; Navasa, Nicolas; Yang, Xiuli; Wilder, Cara N.; Buyuktanir, Ozlem; Marques, Adriana; Pal, Utpal (Cell Press, 2016)Evolution of hematophagy in blood-sucking parasites likely involves communication with their hosts. We find that Ixodes ticks are responsive to IFNg acquired in a blood meal from mice infected with the Lyme disease-causing ... -
Detection of Brucella Antibody and DNA in Cow Milk by ELISA and PCR Methods
Terzi, Goknur; Buyuktanir, Ozlem; Genc, Oktay; Gucukoglu, Ali; Yurdusev, Nevzat (Kafkas Univ, Veteriner Fakultesi Dergisi, 2010)The aim of the present study was to determine comparatively the presence of anti-Brucella antibody and Brucella DNA in cow milk. Anti-Brucella antibody was detected by ELISA based on the lipopolysaccharide (LPS) as diagnostic ... -
Development and validation of sandwich quantitative ELISA prototype based on the bovine IFNg for the detection of cellular immunity
Genc, Oktay; Buyuktanir, Ozlem; Serdar, Gulnur; Kilicoglu, Yunus; Otlu, Salih (Scientific Technical Research Council Turkey-Tubitak, 2015)Immunodiagnostic tests based on in vitro enzymatic methods are mostly preferred to in vivo methods for the detection of cellular immunity due to their cost, applicability, repeatability, and higher sensitivity. Therefore, ... -
Development of an individual rapid test based on enzymatic immunofiltration assay for detection of anti-Brucella abortus antibody in bovine sera
Genc, Oktay; Buyuktanir, Ozlem; Yurdusev, Nevzat (Sage Publications Inc, 2011)To detect bovine antibody directed to smooth lipopolysaccharide (LPS), cell lysate (LYS), O-polysaccharide (OPS), and LPS-deprived chromatographic fractions (ChF) of Brucella abortus, 2 bi-antigenic diagnostic models based ... -
Development of Qualitative and Quantitative ELISA Models for Bovine Brucellosis Diagnosis
Genc, Oktay; Buyuktanir, Ozlem; Yurdusev, Nevzat (Kafkas Univ, Veteriner Fakultesi Dergisi, 2010)In this study, a qualitative and a quantitative ELISA models (iELISA and qELISA, respectively) have been developed for screening and quantifying IgG isotype anti-Brucella abortus LPS antibody. For developing high sensitive ... -
Generation of Mouse Hybridomas Secreting Anti-Salmonella Enteritidis Antibodies and Their Preliminary Characterization
Buyuktanir, Ozlem; Yagci, Tamer; Findik, Arzu; Yildirim, Tuba; Yurdusev, Nevzat (Kafkas Univ, Veteriner Fakultesi Dergisi, 2011)BALB/c mice were intraperitoneally immunized with inactivated bacteria for generation of monoclonal anti-S. Enteritidis antibody. The spleen cells of the highest responder animal at fifth immunization were used as the ... -
HtrA, a Temperature- and Stationary Phase-Activated Protease Involved in Maturation of a Key Microbial Virulence Determinant, Facilitates Borrelia burgdorferi Infection in Mammalian Hosts
Ye, Meiping; Sharma, Kavita; Thakur, Meghna; Smith, Alexis A.; Buyuktanir, Ozlem; Xiang, Xuwu; Pal, Utpal (Amer Soc Microbiology, 2016)High-temperature requirement protease A (HtrA) represents a family of serine proteases that play important roles in microbial biology. Unlike the genomes of most organisms, that of Borrelia burgdorferi notably encodes a ... -
LPS and Flagellin-Based Models for Serological Screening and Confirmation of Salmonella Infections in Chickens
Findik, Arzu; Buyuktanir, Ozlem; Yurdusev, Nevzat (Kafkas Univ, Veteriner Fakultesi Dergisi, 2010)Avian salmonellosis due to Salmonella B and D serogroups has a great importance because of the public health and the economical losses in poultry industry. For accurate diagnosis of avian salmonellosis, a total of 126 ... -
Middle region of the Borrelia burgdorferi surface-located protein 1 (Lmp1) interacts with host chondroitin-6-sulfate and independently facilitates infection
Yang, Xiuli; Lin, Yi-Pin; Heselpoth, Ryan D.; Buyuktanir, Ozlem; Qin, Jinhong; Kung, Faith; Pal, Utpal (Wiley, 2016)Borrelia burgdorferi surface-located membrane protein 1, also known as Lmp1, has been shown to play critical roles in pathogen evasion of host-acquired immune defences, thereby facilitating persistent infection. Lmp1 ... -
Production, Purification and Characterization of the Recombinant Brucella abortus rP17 Protein
Buyuktanir, Ozlem; Genc, Oktay; Yurdusev, Nevzat (Kafkas Univ, Veteriner Fakultesi Dergisi, 2011)Immunoreactive cytosolic P17 protein of Brucella abortus was produced in Escherichia coli as 6xHistidine tagged recombinant protein (rP17) by cloning the p17 gene into pColdI cold-shock expression vector. DNA sequence ... -
Rapid Immunofiltration Assay As A Field Diagnostic Tool For Ovine Brucellosis
Buyuktanir, Ozlem; Genc, Oktay; Celebi, Ozgur; Yurdusev, Nevzat (Taylor & Francis Inc, 2012)This work describes the development of two rapid immunofiltration assays, enzymatic (ERIFA) and non-enzymatic (NERIFA), for the rapid detection of ovine anti-Brucella antibodies. Brucella abortus lipopolysaccharide and ... -
Rapid immunofiltration assay based on colloidal gold-protein G conjugate as an alternative screening test for bovine and ovine brucellosis
Genc, Oktay; Buyuktanir, Ozlem; Yurdusev, Nevzat (Springer, 2012)A non-enzymatic rapid immunofiltration assay (NERIFA) was developed as an alternative field test for rapid detection of anti-Brucella antibody in bovine and ovine sera. The assay was based on Brucella abortus lipopolysaccharide ... -
Serologic detection of antibodies against Fasciola hepatica in sheep in the middle Black Sea region of Turkey
Acici, Mustafa; Buyuktanir, Ozlem; Bolukbas, Cenk Soner; Pekmezci, Gokmen Zafer; Gurler, Ali Tumay; Umur, Sinasi (Elsevier Taiwan, 2017)Background/Purpose: The aim of the present study was to estimate the prevalence of Fasciola hepatica infection in sheep in the Black Sea region of Turkey. Methods: Samples from 213 sheep were collected randomly in Samsun, ... -
Seroprevalence of Fasciolosis in Equines of the Black Sea Region, Turkey
Acici, Mustafa; Bolukbas, Cenk S.; Gurler, Ali T.; Umur, Sinasi; Buyuktanir, Ozlem (Elsevier Science Inc, 2013)This study determined the seroprevalence of fasciolosis in equines (n = 140) by enzyme-linked immunosorbent assay and Western blot. Blood samples were collected from different locations of the Black Sea Region of Turkey. ...
