Nonomuraea jabiensis sp. nov., Isolated from Arid Soil

Abstract

A novel actinomycete, strain A4036T, was isolated from a soil sample collected from the Jabi district in Abuja, Nigeria. The taxonomic position of strain A4036T was established using a combination of genotypic and phenotypic analyses. The organism formed extensively branched substrate and aerial hyphae that generated spiral chains of spores with warty surfaces. The cell wall contained meso-diaminopimelic acid and the cell-wall sugars were glucose, madurose, mannose and ribose. The predominant menaquinone was MK-9(H<inf>4</inf>). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylmethylethanolamine, phosphatidylinositol mannoside, hydroxy-phosphatidyl- ethanolamine, hydroxy-phosphatidylmethylethanolamine, two unidentified phospholipids and four unknown glucosamine-containing phospholipids. The major cellular fatty acids were iso-C<inf>16: 0</inf> 2- OH, iso-C<inf>16: 0</inf> and 10-methyl C<inf>17: 0</inf>. On the basis of 16S rRNA gene sequence similarity studies, strain A4036T grouped in the genus Nonomuraea, being most closely related to Nonomuraea angiospora IFO 13155T (99.05%), Nonomuraea candida HMC10T (98.78%), Nonomuraea kuesteri GW 14-1925T (98.49%), Nonomuraea endophytica YIM 65601T (98.42%), Nonomuraea maheshkhaliensis 16-5-14T (98.40%), Nonomuraea turkmeniaca DSM 43926T (98.38%), Nonomuraea helvata IFO 14681T (98.29%), Nonomuraea rubra DSM 43768T (98.10%) and Nonomuraea salmonea DSM 43678T (98.06%). Levels of 16S rRNA gene sequence similarity to the type strains of other species of the genus Nonomuraea were,98%. Despite the high 16S rRNA gene sequence similarities, DNA-DNA relatedness values and phenotypic data demonstrated that strain A4036T was clearly distinguished from all closely related species of the genus Nonomuraea. Thus, this isolate is considered to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea jabiensis sp. nov. is proposed. The type strain is A4036T (5DSM 45507T5KCTC 19870T). © 2013 IUMS.