Antiadhesive Effects of Mitomycin C and Streptopeptidase A in Rats with Intraperitoneal Adhesions

Abstract

Intraabdominal adhesion formation is a frequent problem after major abdominal surgery. For many years, there have been various attempts to decrease adhesions by using systemic and local drugs and mechanical barriers. In this study we aimed to evaluate the antifibrinolytic antiadhesive effects of mitomycin C (MMC) and streptopeptidase A (SA) against intraabdominal adhesions. Forty-eight rats were divided into six groups, each with eight rats. Group 1 (sham group) rats were laparotomized by transverse incision only. In Group 2 (laparotomy and talcum powder), 2 ml talcum powder was scattered equally onto the intestinal surface after laparotomy. Group 3 (SA only), 2 g SA was introduced onto the intestinal surface. Group 4 (talcum powder and SA), 2 ml talcum powder was scattered onto the intestinal surface and then 2 g SA was applied on the same area. Group 5 (MMC only), 2 ml MMC was introduced onto the intestinal surface. Group 6 (talcum powder and MMC), 2 ml talcum powder was scattered onto intestinal surface and then MMC was applied onto same area. We assessed adhesion grades macroscopically, as well as, hydroxproline levels biochemically. Macroscopicaly, the number of rats with moderate or severe adhesions was significantly higher in the control group than all other groups (P < 0.05). SA and MMC groups had only mild adhesions. No intraabdominal problem was detected in rats with SA or MMC. Hydroxyproline (HP) levels were significantly higher in control group than all other groups (P < 0.05). There was no statistical significance between the rats with SA and MMC (P > 0.05) according to the HP measurements. MMC and SA may have potential antiadhesive effects. Both substances could be beneficial against adhesion formation after laparotomies. © 2007 Springer-Verlag.