Antimicrobial Activity of Thymus leucotrichus and Origanum laevigatum

Abstract

The in vitro antimicrobial activity of Thymus leucotrichus and Origanum laevigatum leaf and extracts were studied against selected fungi and bacteria, following disc diffusion method. Leaves were extracted using ethanol and lyophilized. Remaining solid were dissolved in water or carbon tetrachloride. From these solutions, 3 different concentrations were applied to disk (100, 150, 200 mu g/disc). The water solutions of extracts of both plants were found to be more effective against bacteria. The carbon tetrachloride solutions of extracts were ineffective in inhibiting the bacterial growth or showed poor inhibition. The inhibition zone diameter was seen to increase with the concentration. These results were compared with results obtained using standard antibiotics, imipenem and ampicilin which served as a reference for inhibition zone diameter.