Diagnostic value of ischaemia-modified albumin in pulmonary contusion in rats

Abstract

Background: Patients with pulmonary contusion (PC) are at increased risk of development of complications and death after trauma. The early diagnosis and determination of severity of PC could improve clinical outcomes. The aim of the study was to determine the diagnostic value of ischaemia-modified albumin (IMA) in a PC model in rats. Methods: Thirty-two Sprague-Dawley rats were randomly allocated to four groups; the uninjured control Group I (n = 7) and the uninjured control Group II (n = 7) were euthanised at 2 and 6 h, respectively, and PC groups III (n = 9) and IV (n = 9) were euthanised at 2 and 6 h after trauma, respectively. The serum level of IMA, tissue and serum malondialdehyde (MDA) levels, and histopathological damage scores of the lung tissue were determined. Results: Serum IMA and lung tissue MDA levels in the PC groups were not significantly different to those of the control groups (p = 0.555; p = 0.086, respectively). Serum MDA levels were significantly higher in the PC groups than in the control groups (p = 0.011). When histopathological changes in lung parenchyma were evaluated, there was a statistical difference between the injured and uninjured groups for inflammation and lung injury (p = 0.017; p = 0.001, respectively). However, there was no significant correlation between the histopathological score and biochemical parameters. Conclusion: Our preliminary findings suggest that there is no significant change of serum IMA levels in the acute phase of PC induced by blunt chest trauma. (C) 2011 Elsevier Ltd. All rights reserved.