Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC

Abstract

The aim of this study was to examine the presence of aflatoxin M1 (AFM1) in butter samples marketed in the Black Sea Region of Turkey with two complementary analytical methods to improve accuracy. A total of 40 samples were analysed by enzyme-linked immuno absorbent assay (ELISA) as the screening test and high performance liquid chromatography (HPLC) with fluorescence detection (FLD) after immunoaffinity column (IAC) clean-up as the confirmatory method. Results indicated that butter samples in the Black Sea region were of good quality with respect to AFM1, with no contamination detected. Furthermore, the accurate and sensitive IAC/HPLC-FLD method was confirmed as being appropriate for the detection of AFM1 in butter.