The effect of capsaicin on inducible nitric oxide synthase in puberty and adult rats' ovaries

Abstract

The purpose of this study is the in vivo analysis of the changes in iNOS expression in the ovaries of the capsaicin-treated rats in puberty and adult periods. Rats were divided into with two age groups i.e. puberty (42 days old) and adult (70 days old). Each age group was divided into three sub-groups as treated experimental or vehicle, and untreated control. Starting from 21 days up to 42 and 70 days, implementations were as follows: experimental group received a daily subcutaneous injection of capsaicin (1mg/kg body weight); vehicle group received the vehicle solution (10% Tween 80, 10% ethanol, 80% distilled water) in same volume as experimental group, and untreated control group was not subjected to any injections. In ovaries of all groups, iNOS expression was shown in granulosa cells, interstitial cells, theca follicle cells and corpus luteum luteal cells. An immune reaction was observed in pubertal and adult rat ovaries and iNOS was not inactivated with age. The reaction density was reduced in the experimental groups and showed long-term capsaicin implementation in low dosage negatively affects iNOS stimulation in ovaries.