Molecular identification and phylogeny of bovine herpesvirus-1 (BoHV-1) from cattle associated with respiratory disorders and death in Turkey

Abstract

This study aimed to investigate the presence of bovine herpesvirus 1 by molecular techniques in two cases of respiratory disease in beef cattle, reported from Amasya Province of Turkey in 2018. Nasal swab and lung tissue samples were taken. The presence of bovine herpesvirus-1 (BoHV-1) was confirmed by the PCR method using glycoprotein B (gB) gene-specific primers, and then the isolates were also subjected to partial sequencing. The results of the phylogenetic analysis revealed that the two new isolates in Turkey belonged to the same subclade as subtype 1.1 of BoHV-1, and both also had a 100% nucleotide (nt) homology with the Cooper reference strain of BoHV-1. These findings can enrich the gB sequence content data for BoHV-1 found in GenBank regarding Turkey.