dc.contributor.author | Yılmaz, Veysel T. | |
dc.contributor.author | Icsel, Ceyda | |
dc.contributor.author | Batur, Jenaidullah | |
dc.contributor.author | Aydinlik, Seyma | |
dc.contributor.author | Cengiz, Murat | |
dc.contributor.author | Büyükgüngör, Orhan | |
dc.date.accessioned | 2020-06-21T13:18:58Z | |
dc.date.available | 2020-06-21T13:18:58Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1477-9226 | |
dc.identifier.issn | 1477-9234 | |
dc.identifier.uri | https://doi.org/10.1039/c7dt01286a | |
dc.identifier.uri | https://hdl.handle.net/20.500.12712/12362 | |
dc.description | Yilmaz, Veysel/0000-0002-2849-3332 | en_US |
dc.description | WOS: 000404467500017 | en_US |
dc.description | PubMed: 28607988 | en_US |
dc.description.abstract | A series of new silver(I) 5,5-diethylbarbiturate (barb) complexes with the formulas [Ag-2(mu-barb)(2)(PPh3)(2)] (1), [Ag(barb)(PPh2Cy)] (2), [Ag(barb)(PPhCy2)] (3) and [Ag(barb)(PCy3)] (4) (PPh3 = triphenylphosphine, PPh2Cy = diphenylcyclohexylphosphine, PPhCy2 = dicyclohexylphenylphosphine and PCy3 = tricyclo-hexylphosphine) were synthesized and fully characterized by elemental analysis, IR, NMR, ESI-MS and X-ray crystallography. All the complexes display a significant affinity towards DNA with a groove binding mode and also strongly bind to BSA via hydrophobic interactions. Lipophilicity increases from 1 to 4 with an increasing number of Cy groups in the phosphine ligands. Screening of the in vitro antimicrobial activity of 1-4 against the strains of Gram-negative (S. typhimurium ATCC 14028, E. coli ATCC 25922 and O157:H7) and Gram-positive (L. garvieae 40456, S. aureus ATCC 25923, and ATCC 33591) bacteria demonstrated that all the complexes exhibit very high activity and specific selectivity against the Gram-positive bacteria, compared to AgNO3 and silver sulfadiazine. Furthermore, the growth inhibitory effects of 1-4 on four human cancer cell lines (MCF-7, PC-3, A549 and HT-29) showed that 4 has a potent cytotoxic activity against MCF-7 cells, significantly higher than cisplatin and carboplatin. The effects of the complexes on the inhibition of the cells are closely related to their lipophilicity as well as DNA/protein binding. The induction of apoptosis of MCF-7 cells treated with 4 was probed through Hoechst 33342 staining, Annexin V positivity and caspase 3/7 activity. In addition, increased ROS levels in the presence of 4 are most likely responsible for damage to both mitochondria and genomic DNA. | en_US |
dc.description.sponsorship | Uludag UniversityUludag University; OUAP [F-2016/9] | en_US |
dc.description.sponsorship | This work is a part of a research project, OUAP (F-2016/9). The authors are thankful to Uludag University for the financial support given to the project. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Royal Soc Chemistry | en_US |
dc.relation.isversionof | 10.1039/c7dt01286a | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.title | Synthesis, structures and biomolecular interactions of new silver(I) 5,5-diethylbarbiturate complexes of monophosphines targeting Gram-positive bacteria and breast cancer cells | en_US |
dc.type | article | en_US |
dc.contributor.department | OMÜ | en_US |
dc.identifier.volume | 46 | en_US |
dc.identifier.issue | 25 | en_US |
dc.identifier.startpage | 8110 | en_US |
dc.identifier.endpage | 8124 | en_US |
dc.relation.journal | Dalton Transactions | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |